“Exploring the Interface of LC-MS: Leveraging the Power of Mass Spectrometry for Liquid Chromatography” This comprehensive course will cover the application of mass spec- trometry to enhance the utility of Liquid Chromatography, focusing on application and troubleshooting. The course will be taught by Dr. Martha M. Vestling, The Director of the UW-Madison Mass Spectrometry Center. “Mass Spectrometry for Chromatographers" This exciting, immersive course illustrates the power and utility of mass spectrometry as a hyphenated technique in conjunction with Gas Chromatography for identifying unknown compounds that may not be identified through traditional chemometric approaches. The course will be taught by Dr. Robert J. Kobelski, the Lead Chemist at Resolution Sciences. “Optimizing GC Methods - What do you want...Sensitivity, Resolution or Speed?” This practical, comprehensive two-day course focuses systematically on methodologies to improve GC performance. The course will cover the major aspects of GC method development and how they can be fine-tuned to find a balance between speed and resolution. The course will be taught by Daron Decker the Gas Chromatography Applications Specialist at Agilent Technologies.
All courses will be held at the Earle Browne Center on May 5th and 6th. Cost is $575 Registration coming soon. Questions? Please email Christian Toonstra at (toonstrac@gmail.com) Please see below for more details pertaining to our exciting courses!
“Exploring the Interface of LC-MS: Leveraging the Power of Mass Spectrometry for Liquid Chromatography”
Course Description This 1.5-day course will show how mass spectrometers can be great detectors for goodchromatography. The challenge for massspectrometry is to put molecules/compounds exitingchromatographs into the gas phase as ions. Mass spectra then provide 1) masses of ions, 2)isotope patterns of ions, 3) masses and isotope patterns of fragments of ions, and 4) the resolving powers of the mass spectrometers used. When mixtures are analyzed, it is not theleast bit unusual for one component to be much more easily ionized than the others which can result in ion suppression. When chromatography is used to separate mixtures, mass spectrometry can analyze more of the components present. Chromatography with diode arraydetection and mass spec detection generates multiple characteristic numerical measurements of mixtures (data), helping to confirm identifications and the amounts present. To highlight concepts covered in this course, a collection of less-than-successful LC/MS experiments (carried out on functioning instrumentation) will be discussed. Course Topics: mass calculations, ionization methods, MS/MS (tandem mass spectrometry), analyzers, GC/MS, LC/MS, bottom-up, top-down, quantitation, surfaces and imaging, ion mobility and databases.
Martha M. Vestling, Ph.D.: Senior Scientist and Director of the UW-Madison MS Facility Instructor Biography Dr. Martha M. Vestling is a Senior Scientist and Director of the Chemistry Instrument Center’s Mass Spectrometry Facility at the University of Wisconsin-Madison. Currently the lab features seven mass spectrometers with four types of ionization available: EI, ESI, MALDI, and ASAPMS ™. One of the instruments has a gas chromatograph in front of it, while four have UHPLCs attached. Located in the Chemistry Department, the lab sees all types of samples ranging from hydrocarbons to organometallics to non-natural peptides to proteins to synthetic intermediates and products. After training, graduate students and postdocs can operate five of the seven spectrometers and develop specific GC/MS and LC/MS methods for their research projects. Martha has a BA in chemistry from Oberlin College and a PhD in chemistry from Northwestern University. Her career includes stops at the University of Florida (postdoc), Vanderbilt University (postdoc), State University of New York College at Brockport (faculty), University of Illinois (sabbatical), University of Maryland Baltimore County (sabbatical then visitor) and time between the University of Delaware and DuPont Central Research (visitor) before moving to Madison, WI over twenty years ago.
“Mass Spectrometry for Chromatographers"
Course Description
When three of the Four Norsemen, a Bemidji curling team, die of an apparent alcohol related exposure and the BCA finds an uncharacterized peak in the GC-TCD blood alcohol analysis the samples are transferred to a GC-MS analyst. The samples are subjected to headspace GC-EIMS instrument for the qualitative, and subsequent quantitative analysis of the unidentified peak. In this section we will explore the fundamentals of a mass spectrum, the most common GC-MS interface, Electron Ionization, quadrupole mass analyzers, an introduction to qualitative analysis of a mass spectrum, and the basic rules for quantitative analysis by mass spectrometry. Harley Chapman, the Chemistry Department stockroom attendant, is found dead in the stockroom early on Monday morning at the Heisenberg Institute of Technology in Albuquerque, NM. Walter Blanco of the NM State Crime Lab analyzes both a urine sample as well as a white powder, with a bluish tint, found in and around a broken 10 dram vial near the corpse by GC-MS. Unfortunately the EIMS of the unknown yields a spectrum with little qualitative utility. Dr. White moves the samples to the lab’s ESI-LC-MS/MS to obtain molecular weight information as well as fragmentation from collisionally induced dissociation to identify the toxic agent. In this section we will explore; the LC-MS interfaces, soft ionization techniques including Electrospray Ionization (+ and – ESI) and Atmospheric Chemical Ionization, as well as the utility of tandem mass spectrometry (“triple quad” and Q-TOF). This course assumes an understanding of basic chromatographic concepts and requires willing suspension of disbelief with respect to the absence of spectra for the toxic agents in the NIST library of mass spectra.
Robert J. Kobelski, Ph.D.: Principal Scientist at Resolution Sciences Instructor Biography Dr. Kobelski’s career as an analytical chemist has spanned more than 35 years in a variety of roles and environments. From bench positions in private industry to a leadership role in government he has been driven by his desire to solve problems through chemical analysis and transfer the techniques and technologies for problem solving to others. At CDC he was responsible for developing high throughput clinical analysis methods, creating a mechanism for training more than 40 public health laboratories in the performance of those methods and establishing and maintaining a proficiency testing program to demonstrate the lab network’s capability.
“Optimizing GC Methods - What do you want...Sensitivity, Resolution or Speed?”
Course Description This course will look at the major areas in GC method development for optimum results. Choosing the carrier gas type and controlling the velocity for maximum resolution or speed will be discussed. Injection modes and parameters will be explored for optimum peak shape and maximum sensitivity for sample introduction. Selecting the GC column stationary phase and dimensions as to how they affect peak shape, resolution and analysis time. Specific instruction will be given on how to best manipulate the oven temperature program to maximize resolution or reduce overall analysis times.
Daron Decker: Gas Chromatography Applications Specialist at Agilent Technologies Instructor Biography Daron Decker is a Gas Chromatography Applications Specialist for the Chemistries and Supplies Division at Agilent Technologies. Prior to Agilent, Daron worked for nearly a decade for J&W Scientific in the area of technical support. Daron has given many seminars, courses and technical papers on GC both domestic and international. Daron’s seminars are well known in the industry for being excellent, informative and entertaining! In May 2003, Daron was awarded the Palmer Award by the MCF. Daron has over 30 years of GC experience and currently lives in the Houston, TX area with his wife of 31 years.