Introduction to (U)HPLC
Introduction to (U)HPLC
This is a hybrid course consisting of both video content (complete before June 11, at your own pace), and live, in-person training. The total content is equivalent to a 1.5-day course. Participants will receive access to 5.5 hours of video-based training content through the Analytical Training Solutions platform, which includes several brief assessments following each training module. Course topics include: history of HPLC, operating principles of key instrument components (pumps, samplers, etc.), detection principles, and more.
This is a hybrid course consisting of both video content (complete before June 11, at your own pace), and live, in-person training. The total content is equivalent to a 1.5-day course.
Video Component – Participants will receive access to 5.5 hours of video-based training content through the Analytical Training Solutions platform, which includes several brief assessments following each training module. This content and the assessments should be completed prior to June 11th.
Live, In-Person Component – This in-person session will be held 8:30 to 2:00 PM on Wednesday, June 11th. Activities will include: 1) Opportunities for Q&A and discussion in response to participant questions following the video modules; 2) Problem-solving exercises facilitated by the instructor to reinforce key concepts; 3) Demonstration of web-based tools for learning about HPLC and method development; and 4) Hands-on demonstration of key components including fittings, columns, and tubing.
Course Topics:
- History of HPLC
- Operating principles of key instrument components (pumps, samplers, etc.)
- Detection principles
- Key definitions and performance metrics, including retention, selectivity, symmetry, and efficiency
- Different modes of LC (reversed-phase, normal phase, etc.)
- Gradient elution separations
- Principles of peak integration
- Principles of quantitation
- Survey of common problems
Professor of Chemistry at Gustavus Adolphus College
Dwight Stoll is Professor of Chemistry at Gustavus Adolphus College in St. Peter, MN. He has authored or co-authored more than 99 peer-reviewed publications and six book chapters in separation science, and speaks internationally on the topic. He is co-editor of the recently released book on “Multi-Dimensional Liquid Chromatography: Principles, Practice, and Applications”. He has also written more than 70 of the monthly “LC Troubleshooting” articles for LCGC Magazine since 2017, and has co-hosted the “Analytically Speaking” podcast since 2022. His primary research focus is on the development of two-dimensional liquid chromatography (2D-LC) for both targeted and untargeted analyses. Within this area he has made contributions on many aspects of the technique including stationary phase characterization, biopharmaceutical analysis, new 2D-LC methodologies and instrumentation, and fundamental aspects including re-equilibration in gradient elution liquid chromatography and analyte focusing. He regularly teaches short courses on chromatography in general, and 2D-LC, specifically, including venues such as Pittcon, EAS, and the international HPLC20XX series. He is the 2011 recipient of LCGC’s Emerging Leader in Chromatography Award, and the 2015 recipient of the American Chemical Society Division of Analytical Chemistry Award for Young Investigators in Separation Science.
Introduction to (U)HPLC
This is a hybrid course consisting of both video content (complete before June 11, at your own pace), and live, in-person training. The total content is equivalent to a 1.5-day course. Participants will receive access to 5.5 hours of video-based training content through the Analytical Training Solutions platform, which includes several brief assessments following each training module. Course topics include: history of HPLC, operating principles of key instrument components (pumps, samplers, etc.), detection principles, and more.